e study  together with other bacteria/proteins was performed. For this, the cell lysates (1 μg each) of various Aeromonas strains were blotted onto a nitrocellulose membrane using a slot blotter (cleaver scientific Ltd, UK) and developed in a manner similar to western blot assay.. Four different sample number size and styles of Hybridisation Manifold are offered: 48 or 96 well dot blotter and 24 or 48 well slot blotters. Machined from high density acrylic with precision lapped mating surfaces to ensure uniform filter contact, these manifolds are designed for DNA and RNA membrane hybridisations and antigen/antibody screening in immunological applications. Each manifold consists of paired upper dot- or slot-forming modules and a lower vacuum chamber module. A vacuum of approximately mm Hg ( bar) is Brand: VWR®.
Dot and Slot Blotters
These prevent lateral transfer of samples — smudging — by ensuring that a complete vacuum is formed. Place 3 presoaked blotting papers onto the top of the manifold's middle plate. Log in. A vacuum of approximately mm Hg 0. Representative data Figure 3. Once your questions are answered, you will be informed using the email address that you register with bio-protocol.
dotBLOT and slotBLOT
A well performing QC laboratory ensures the integrity of a company's production process from raw material validation to finished product verification Our goal is to provide you with the most up to date information on solutions VWR is already a major supplier of specialised staining materials for the Pathology market.
We are building our product portfolio for our clinical customers daily VWR offers a wide range of solutions for microbiological control dedicated to the Industrial market sector A large choice of products and services for complete water analysis on-site and in the laboratory Avantor Services provides a wide range of specialized services and digital solutions to help you solve complex challenges.
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Four different sample number, size and style of Hybridisation Manifold are offered; two types of Dot Blotter and two types of Slot Blotter. The units incorporate precision lapped surfaces to ensure uniform blotting membrane contact and a leak proof gasket.
These prevent lateral transfer of samples and smudging by ensuring that a complete vacuum is formed. Dot Blotters are available in 48 and 96 well versions and Slot Blotters in 24 and 48 well.
This unit has a 12 month limited warranty, for more information, consult our terms and conditions. Key Features Low cost Simple construction Easy assembly Four sample configurations Alpha-numeric sample identification.
Add to Cart. Special Requirements? Get a Custom Quote. Catalogue Page Number: 45 This unit has a 12 month limited warranty, for more information, consult our terms and conditions. This product has been cited in the following research papers: Reference title Date Citation Product usage A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration Ganguli, N.
Appl Microbiol Biotechnol Immunol Res The membranes were prehybridized for 4 hours, followed by hybridization with respective transgene specific probes for 10—12 hours. The hybridized bl… Characterization of immune response elicited by the recombinant outer membrane protein OmpF of Aeromonas hydrophila, a potential vaccine candidate in murine model Yadav, S.
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